Difference between revisions of "Part:BBa K197028:Design"

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Ost006R  Reverse oligo for Beta Helix Repeats
 
Ost006R  Reverse oligo for Beta Helix Repeats
 
ctagcGGATCCtgtacgcatgacaaatgctgac
 
ctagcGGATCCtgtacgcatgacaaatgctgac
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+
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===References===
 
===References===

Revision as of 04:17, 22 October 2009

Beta Helix


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This is a beta helix


Source

Construction of Beta Helix Repeats basic part
PCR Ost005F and Ost006R on E. coli avian APEC genomic DNA (703bp, EcoRI/BamHI)
Sub into JCA_pBca9495AK-Bca1144      (EcoRI/BamHI, 3171+910, L)
Product is JCA_pBca9495AK-M10042     {Beta Helix Repeats}
----------------------------
Ost005F   Forward oligo for Beta Helix Repeats
ccaaaGAATTCatgAGATCTagtgtcttcaacggcaccg
Ost006R   Reverse oligo for Beta Helix Repeats
ctagcGGATCCtgtacgcatgacaaatgctgac

References