Difference between revisions of "Part:BBa K5106009"
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This miRNA acts as one of the two trigger RNAs. It will anneal with the other miRNA due to partial complementarity. They form a structure together which will trigger the AND gate toehold switch. This triggering results in the expression of the output gene. The absence of this miRNA leads to no expression of the output gene. | This miRNA acts as one of the two trigger RNAs. It will anneal with the other miRNA due to partial complementarity. They form a structure together which will trigger the AND gate toehold switch. This triggering results in the expression of the output gene. The absence of this miRNA leads to no expression of the output gene. | ||
| − | + | ==Usage and Biology== | |
| − | + | This part is used in ''in vitro'' experiments, to test the functionality of the <span class="plainlinks">[https://parts.igem.org/Part:BBa_K5106007 toehold AND gate]</span>. This gate gets activated by two trigger RNA, of which this one is the second, and <span class="plainlinks">[https://parts.igem.org/Part:BBa_5106008 BBa_5106008]</span> is the first. ''In vitro'' experiments were performed on the composite part <span class="plainlinks">[https://parts.igem.org/Part:BBa_K5106010 BBa_K5106010]</span>, where toehold switch is under a T7 promoter and terminator, regulating LacZ. For more information on these parts, please take a look at <span class="plainlinks">[https://2024.igem.wiki/wageningenur miRADAR (WangeningenUR 2024)]</span> | |
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| − | <span class='h3bb'>Sequence and Features</span> | + | =<span class='h3bb'>Sequence and Features</span>= |
<partinfo>BBa_K5106009 SequenceAndFeatures</partinfo> | <partinfo>BBa_K5106009 SequenceAndFeatures</partinfo> | ||
Latest revision as of 20:55, 1 October 2024
Trigger 2 for toehold AND gate
This miRNA acts as one of the two trigger RNAs. It will anneal with the other miRNA due to partial complementarity. They form a structure together which will trigger the AND gate toehold switch. This triggering results in the expression of the output gene. The absence of this miRNA leads to no expression of the output gene.
Usage and Biology
This part is used in in vitro experiments, to test the functionality of the toehold AND gate. This gate gets activated by two trigger RNA, of which this one is the second, and BBa_5106008 is the first. In vitro experiments were performed on the composite part BBa_K5106010, where toehold switch is under a T7 promoter and terminator, regulating LacZ. For more information on these parts, please take a look at miRADAR (WangeningenUR 2024)
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]