Difference between revisions of "Part:BBa K197028:Design"
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<partinfo>BBa_K197028 short</partinfo> | <partinfo>BBa_K197028 short</partinfo> | ||
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===Source=== | ===Source=== | ||
− | + | <pre> | |
− | + | Construction of Beta Helix Repeats basic part | |
+ | PCR Ost005F and Ost006R on E. coli avian APEC genomic DNA (703bp, EcoRI/BamHI) | ||
+ | Sub into JCA_pBca9495AK-Bca1144 (EcoRI/BamHI, 3171+910, L) | ||
+ | Product is JCA_pBca9495AK-M10042 {Beta Helix Repeats} | ||
+ | ---------------------------- | ||
+ | Ost005F Forward oligo for Beta Helix Repeats | ||
+ | ccaaaGAATTCatgAGATCTagtgtcttcaacggcaccg | ||
+ | Ost006R Reverse oligo for Beta Helix Repeats | ||
+ | ctagcGGATCCtgtacgcatgacaaatgctgac | ||
+ | <pre> | ||
===References=== | ===References=== |
Revision as of 04:17, 22 October 2009
Beta Helix
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This is a beta helix
Source
Construction of Beta Helix Repeats basic part PCR Ost005F and Ost006R on E. coli avian APEC genomic DNA (703bp, EcoRI/BamHI) Sub into JCA_pBca9495AK-Bca1144 (EcoRI/BamHI, 3171+910, L) Product is JCA_pBca9495AK-M10042 {Beta Helix Repeats} ---------------------------- Ost005F Forward oligo for Beta Helix Repeats ccaaaGAATTCatgAGATCTagtgtcttcaacggcaccg Ost006R Reverse oligo for Beta Helix Repeats ctagcGGATCCtgtacgcatgacaaatgctgac <pre> ===References===