Difference between revisions of "Part:BBa K5499008"
 
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P2A is a self-cleaving peptide derived from Porcine teschovirus-1, a short peptide (typically consisting of 18-22 amino acids) that mediates the cleavage of polyproteins during translation in eukaryotic cells. The P2A peptide is highly useful in gene therapy and protein expression because it can efficiently cleave multiple gene products into mature proteins without the need for proteases.
 
P2A is a self-cleaving peptide derived from Porcine teschovirus-1, a short peptide (typically consisting of 18-22 amino acids) that mediates the cleavage of polyproteins during translation in eukaryotic cells. The P2A peptide is highly useful in gene therapy and protein expression because it can efficiently cleave multiple gene products into mature proteins without the need for proteases.
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===Usage and Biology===
 
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1. Self-Cleaving Mechanism:
 
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P2A peptide has a specific amino acid sequence that enables it to separate two proteins at the end of translation through an intrinsic self-cleavage mechanism. The process occurs as follows:
 
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·When translating to the P2A peptide, a transient peptide chain is produced.
 
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·At the C-terminus of the P2A peptide, an amide bond breaks, resulting in two independent proteins.
 
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·This cleavage process is enzymatic and does not rely on external enzymes.
 
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2. Translation Efficiency:
 
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The cleavage efficiency of P2A is very high, typically exceeding 90%, meaning most translated mRNA successfully produces two functional proteins. This high efficiency makes P2A widely applicable in genetic engineering.
 
  
 
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<partinfo>BBa_K5499008 parameters</partinfo>
 
<partinfo>BBa_K5499008 parameters</partinfo>
 
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===Profile===
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Name: P2A Peptide
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Base Pairs:66 bp
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Origins: The P2A peptide is derived from the Foot-and-Mouth Disease Virus (FMDV). It is a short, self-cleaving peptide that facilitates the co-expression of multiple proteins from a single mRNA transcript.
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Properties: P2A enables a ribosomal skipping mechanism during translation, allowing for the production of distinct proteins without the need for additional cleavage enzymes. It is characterized by its efficiency in various expression systems, maintaining the integrity and functionality of the resulting proteins. The use of P2A is advantageous in applications requiring the simultaneous expression of multiple proteins, such as in vaccine development and gene therapy.
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===Usage and Biology===
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The P2A peptide is commonly used in synthetic biology for the co-expression of multiple proteins from a single open reading frame. By placing the P2A sequence between coding sequences, researchers can ensure that proteins are produced as separate entities despite being transcribed from the same gene. This technique is particularly useful in applications such as generating multivalent vaccines, producing fusion proteins, and engineering cells for therapeutic purposes.
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P2A is a self-cleaving peptide derived from the Foot-and-Mouth Disease Virus (FMDV). It allows for the ribosomal skipping mechanism during translation, where the ribosome skips the peptide bond formation at the P2A site, leading to the production of distinct protein products. The unique feature of P2A is its ability to function efficiently at various expression levels without significantly affecting the protein folding or activity of the resulting proteins. This makes P2A an invaluable tool in protein engineering and molecular biology.

Latest revision as of 20:19, 1 October 2024


P2A is a self-cleaving peptide

P2A is a self-cleaving peptide derived from Porcine teschovirus-1, a short peptide (typically consisting of 18-22 amino acids) that mediates the cleavage of polyproteins during translation in eukaryotic cells. The P2A peptide is highly useful in gene therapy and protein expression because it can efficiently cleave multiple gene products into mature proteins without the need for proteases.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Profile

Name: P2A Peptide

Base Pairs:66 bp

Origins: The P2A peptide is derived from the Foot-and-Mouth Disease Virus (FMDV). It is a short, self-cleaving peptide that facilitates the co-expression of multiple proteins from a single mRNA transcript.

Properties: P2A enables a ribosomal skipping mechanism during translation, allowing for the production of distinct proteins without the need for additional cleavage enzymes. It is characterized by its efficiency in various expression systems, maintaining the integrity and functionality of the resulting proteins. The use of P2A is advantageous in applications requiring the simultaneous expression of multiple proteins, such as in vaccine development and gene therapy.

Usage and Biology

The P2A peptide is commonly used in synthetic biology for the co-expression of multiple proteins from a single open reading frame. By placing the P2A sequence between coding sequences, researchers can ensure that proteins are produced as separate entities despite being transcribed from the same gene. This technique is particularly useful in applications such as generating multivalent vaccines, producing fusion proteins, and engineering cells for therapeutic purposes.

P2A is a self-cleaving peptide derived from the Foot-and-Mouth Disease Virus (FMDV). It allows for the ribosomal skipping mechanism during translation, where the ribosome skips the peptide bond formation at the P2A site, leading to the production of distinct protein products. The unique feature of P2A is its ability to function efficiently at various expression levels without significantly affecting the protein folding or activity of the resulting proteins. This makes P2A an invaluable tool in protein engineering and molecular biology.