Difference between revisions of "Part:BBa K247004:Experience"

 
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*Time when we observed mRFP fluorescence : 24 h
 
*Time when we observed mRFP fluorescence : 24 h
  
*medium : 10 mL ofLB-Amp,Cm medium containing 1(left) or 5%(right) agar
+
*medium : 10 mL of LB-Amp,Cm medium containing 1(left) or 5(right) %(w/v) agar
  
 
[[Image:Chiba_BBa_K247004.png|thumb|left|'''Fig. 1 RFP Fluorescent'''. RFP expression is induced by AHL (100 nM). Observed under UV irradiation.]]
 
[[Image:Chiba_BBa_K247004.png|thumb|left|'''Fig. 1 RFP Fluorescent'''. RFP expression is induced by AHL (100 nM). Observed under UV irradiation.]]

Latest revision as of 04:09, 22 October 2009

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Applications of BBa_K247004

User Reviews

UNIQae5040224d8c2a21-partinfo-00000000-QINU UNIQae5040224d8c2a21-partinfo-00000001-QINU

This part properly acted as RFP reporter.

Excitation peak: 584 nm, Emission peak: 607 nm


The Chiba iGEM team tested this part in several conditions.

Experiment Condition

  • AHL concentration of medium : 100 nM
  • Time when we observed mRFP fluorescence : 24 h
  • medium : 10 mL of LB-Amp,Cm medium containing 1(left) or 5(right) %(w/v) agar
Fig. 1 RFP Fluorescent. RFP expression is induced by AHL (100 nM). Observed under UV irradiation.