Difference between revisions of "Part:BBa K5401006:Design"
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===Design Notes=== | ===Design Notes=== | ||
No design considerations. | No design considerations. | ||
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===Source=== | ===Source=== | ||
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The sequence was ordered as a gBlock from IDT, together with other elements (ie. ASO targeted against T7RNAP mRNA; and lac promoter). | The sequence was ordered as a gBlock from IDT, together with other elements (ie. ASO targeted against T7RNAP mRNA; and lac promoter). | ||
Latest revision as of 19:43, 1 October 2024
Antisense Oligonucleotide System (ASO)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 141
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
No design considerations.
Source
The sequence was ordered as a gBlock from IDT, together with other elements (ie. ASO targeted against T7RNAP mRNA; and lac promoter).
References
Liang, X., Li, C., Wang, W., & Li, Q. (2018). Integrating T7 RNA Polymerase and Its Cognate Transcriptional Units for a Host-Independent and Stable Expression System in Single Plasmid. ACS Synthetic Biology, 7(5), 1424–1435. https://doi.org/10.1021/acssynbio.8b00055
Vogel, J., & Luisi, B. F. (2011). Hfq and its constellation of RNA. Nature reviews. Microbiology, 9(8), 578–589. https://doi.org/10.1038/nrmicro2615