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Revision as of 19:36, 1 October 2024

This collection is a user contributed collection, and is not under curation by iGEM HQ/Registry.

VEGFR-like

This year, the USTC iGEM teamlianjiewenben has utilized the competitive binding of vascular endothelial growth factor (VEGF) to develop a targeted bacterial therapy for solid tumors. Our quest for the optimal VEGF-binding protein(or peptide) led us to an in-depth exploration of proteins structurally akin to the vascular endothelial growth factor receptor (VEGFR), which we have named VEGFR-like. In this year's project, we selected pBBR1MCS-2 as our plasmid backbone to construct a series of plasmids, which are used to validate the surface display system, VEGFR-like, and the masking peptides targeting VEGFR-like. And we have verified the expression of several of these plasmids in EcN.

Name Type Description Designer Length
BBa_K5302000 Coding VEGFR1D2 Jiacan Ma 303
BBa_K5302001 Coding 8IIU Jiacan Ma 378
BBa_K5302002 Coding 8IJZ Jiacan Ma 378
BBa_K5302003 Coding ZVEGF Dekun Zhou 177
BBa_K5302004 Coding Z3C Dekun Zhou 177
BBa_K5302005 Coding miniZ Dekun Zhou 102
BBa_K5302006 Coding V114 Dekun Zhou 57
BBa_K5302007 Coding V107 Dekun Zhou 57
BBa_K5302008 Coding VEGFR-masking#18 Dekun Zhou 93
BBa_K5302009 Coding VEGFR-masking#29 Dekun Zhou 84
BBa_K5302010 Coding VGB Dekun Zhou 96
BBa_K5302011 Plasmid pBBR-OmpA-mCherry Jiacan Ma 6483
BBa_K5302012 Plasmid pBBR-INP-mcherry Jiacan Ma 6799
BBa_K5302013 Plasmid pBBR-LppOmpA-mCherry Bojun Yang 5940
BBa_K5302014 Plasmid pBBR-intimin-mCherry Bojun Yang 6957
BBa_K5302015 Plasmid pBBR-AIDA-mCherry Bojun Yang 7269
BBa_K5302016 Plasmid pBBR-OmpA-VEGFR1D2 Xinyuan Shi 6150
BBa_K5302017 Plasmid pBBR-OmpA-8IIU Xinyuan Shi 6225
BBa_K5302018 Plasmid pBBR-OmpA-8IJZ Xinyuan Shi 6225
BBa_K5302019 Plasmid pBBR-OmpA-ZVEGF Xinyuan Shi 6024
BBa_K5302020 Plasmid pBBR-OmpA-Z3C Xinyuan Shi 6024
BBa_K5302021 Plasmid pBBR-OmpA-miniZ Xinyuan Shi 5949
BBa_K5302022 Plasmid pBBR-OmpA-V114 Xinyuan Shi 5903
BBa_K5302023 Plasmid pBBR-OmpA-V107 Xinyuan Shi 5903
BBa_K5302024 Plasmid pBBR-INP-VEGFR1D2 Bojun Yang 6466
BBa_K5302025 Plasmid pBBR-INP-8IIU Bojun Yang 6541
BBa_K5302026 Plasmid pBBR-INP-8IJZ Bojun Yang 6541
BBa_K5302027 Plasmid pBBR-INP-ZVEGF Bojun Yang 6340
BBa_K5302028 Plasmid pBBR-INP-Z3C Bojun Yang 6340
BBa_K5302029 Plasmid pBBR-INP-miniZ Bojun Yang 6265
BBa_K5302030 Plasmid pBBR-INP-V114 Bojun Yang 6220
BBa_K5302031 Plasmid pBBR-INP-V107 Bojun Yang 6220
BBa_K5302032 Plasmid pBBR-OmpA-VEGFR1D2-l1masking#18 Dekun Zhou 6144
BBa_K5302033 Plasmid pBBR-OmpA-VEGFR1D2-l2masking#18 Dekun Zhou 6144
BBa_K5302034 Plasmid pBBR-OmpA-VEGFR1D2-l1masking#29 Dekun Zhou 6135
BBa_K5302035 Plasmid pBBR-OmpA-VEGFR1D2-l2masking#29 Dekun Zhou 6135
BBa_K5302036 Plasmid pBBR-OmpA-l1VGB Dekun Zhou 6147
BBa_K5302037 Plasmid pBBR-OmpA-l2VGB Dekun Zhou 6147
BBa_K5302038 Plasmid pBBR-INP-VEGFR1D2-l1masking#18 Dekun Zhou 6496
BBa_K5302039 Plasmid pBBR-INP-VEGFR1D2-l2masking#18 Dekun Zhou 6496
BBa_K5302040 Plasmid pBBR-INP-VEGFR1D2-l1masking#29 Dekun Zhou 6487
BBa_K5302041 Plasmid pBBR-INP-VEGFR1D2-l2masking#29 Dekun Zhou 6487
BBa_K5302042 Plasmid pBBR-INP-l1VGB Dekun Zhou 6499
BBa_K5302043 Plasmid pBBR-INP-l2VGB Dekun Zhou 6499

Reference

[1]Ivan Guryanov*, Viktor Korzhikov-Vlakh, Madhushree Bhattacharya, Barbara Biondi, Giulia Masiero, Fernando Formaggio, Tatiana Tennikova, Arto Urtt. Conformationally Constrained Peptides with High Affinity to the Vascular Endothelial Growth Factor.Journal of Medicinal Chemistry. Vol 64/Issue 15.July 16, 2021
[2]Pan B, Li B, Russell SJ, Tom JY, Cochran AG, Fairbrother WJ. Solution structure of a phage-derived peptide antagonist in complex with vascular endothelial growth factor. J Mol Biol. 2002 Feb 22;316(3):769-87. doi: 10.1006/jmbi.2001.5370
[3]Checco JW, Kreitler DF, Thomas NC, Belair DG, Rettko NJ, Murphy WL, Forest KT, Gellman SH. Targeting diverse protein-protein interaction interfaces with α/β-peptides derived from the Z-domain scaffold. Proc Natl Acad Sci U S A. 2015 Apr 14;112(15):4552-7. doi: 10.1073/pnas.1420380112
[4]Rossella Di Stasi, Donatella Diana, Domenica Capasso, Rosanna Palumbo, Alessandra Romanelli, Carlo Pedone, Roberto Fattorusso, Luca D. D'Andrea. VEGFR1D2 in drug discovery: Expression and molecular characterization.19 November 2010
[5]Anna Fedorova, Kerry Zobel, Herman S. Gill, Annie Ogasawara, Judith E. Flores, Jeff N. Tinianow, Alexander N. Vanderbilt, Ping Wu, Y. Gloria Meng, Simon-P. Williams, Christian Wiesmann, Jeremy Murray, Jan Marik, Kurt Deshayes. The Development of Peptide-Based Tools for the Analysis of Angiogenesis. Chemistry & Biology. Volume 18, Issue 7. 2011. Pages 839-845. ISSN 1074-5521
[6]Balsera B, Bonache MÁ, Reille-Seroussi M, Gagey-Eilstein N, Vidal M, González-Muñiz R, Pérez de Vega MJ. Disrupting VEGF-VEGFR1 Interaction: De Novo Designed Linear Helical Peptides to Mimic the VEGF13-25 Fragment. Molecules. 2017 Oct 28;22(11):1846
[7]Lei Wang,Lingyu Zhou,Marie Reille-Seroussi,Nathalie Gagey-Eilstein,Sylvain Broussy, Tianyu Zhang,Lili Ji,Michel Vidal*,Wang-Qing Liu.Identification of Peptidic Antagonists of Vascular Endothelial Growth Factor Receptor 1 by Scanning the Binding Epitopes of Its Ligands.July7.2017
[8]Sonia Nicchi1,2, Maria Giuliani1 , Fabiola Giusti1 , Laura Pancotto1 , Domenico Maione1 , Isabel Delany1 , Cesira L. Galeotti1 and Cecilia Brettoni1*.Decorating the surface of Escherichia coli with bacterial lipoproteins: a comparative analysis of diferent display systems.2021