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LAMP2A signal peptide

The signal peptide of LAMP2A is a short amino acid sequence (typically around 15-30 residues) that serves as a molecular "address" that directs the protein to the secretory pathway.

Sequence and Features

Profile

Name: LAMP2A Signal Peptide

Base Pairs: 84 bp

Origins: Derived from the lysosomal-associated membrane protein 2 (LAMP2) gene, commonly found in mammals.

Properties: The LAMP2A signal peptide facilitates the targeting of proteins to lysosomes, plays a crucial role in the secretion and membrane integration of lysosomal proteins, and is characterized by its hydrophobic nature that promotes membrane interactions.

Usage and Biology

The LAMP2A signal peptide is commonly used in synthetic biology and genetic engineering to direct proteins to lysosomes. By attaching this signal peptide to the N-terminus of recombinant proteins, researchers can ensure proper localization within the lysosomal compartment, which is essential for the function of lysosomal enzymes and proteins. This application is particularly valuable in therapeutic contexts, such as enzyme replacement therapies for lysosomal storage disorders.

In mammals, the LAMP2A signal peptide is part of the LAMP2 protein, which is integral to lysosomal membrane dynamics. LAMP2 exists in three isoforms (LAMP2A, LAMP2B, and LAMP2C), with LAMP2A being the primary isoform involved in chaperone-mediated autophagy (CMA). This process allows selective degradation of cytosolic proteins through lysosomal pathways, highlighting the peptide's importance in cellular homeostasis and protein turnover. The LAMP2A signal peptide is critical for recognizing and translocating proteins into lysosomes, contributing to the regulation of lysosomal functions.