Difference between revisions of "Part:BBa K5136231"

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<center><html><B>Figure 1 Gene circuit of BBa_K5136231 </B></html></center>
 
<center><html><B>Figure 1 Gene circuit of BBa_K5136231 </B></html></center>
  
===Characterization===
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>

Revision as of 18:34, 1 October 2024


J23106-SD7-LexRO-B0015-pColE408-ccdB-B0014-pHybrid 2)-114 version-B0034-ccdA

Biology

pColE48 is a promoter to which LexRO (BBa_K5136042) binds and represses the expression of genes driven by the promoter. The gene ccdB encodes a toxic protein (CcdB) that, as a DNA gyrase poison, locks DNA gyrase together with broken double-stranded DNA, ultimately leading to cell death. The gene ccdA is found within the ccd operon, encoding the antidote protein (CcdA) that protects cells from the toxic effects of CcdB.Lonprotease easily degrades CcdA protein. The cell loses the ccdA gene due to the loss of the F plasmid, causing the cell to succumb to the toxicity of CcdB.

Usage

To verify the performance of blue light induced kill switch, we designed a composite part: BBa_K5136231.

Figure 1 Gene circuit of BBa_K5136231


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 1607
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 456
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1376