Difference between revisions of "Part:BBa K5499005"

 
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The signal peptide of LAMP2A is a short amino acid sequence (typically around 15-30 residues) that serves as a molecular "address" that directs the protein to the secretory pathway. Here are key roles the LAMP2A signal peptide plays:
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The signal peptide of LAMP2A is a short amino acid sequence (typically around 15-30 residues) that serves as a molecular "address" that directs the protein to the secretory pathway.  
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Translocation to the ER: The signal peptide facilitates the translocation of the LAMP2A precursor protein into the lumen of the ER. This is essential for proper folding and modification (e.g., glycosylation).
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Membrane Integration: After processing in the ER, the signal peptide is cleaved off, and the mature LAMP2A protein is integrated into the lysosomal membrane, where it can perform its functions.
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If you are interested in exosome-based drug delivery, incorporating the LAMP2A signal peptide can help direct proteins into exosomes. This can increase the efficiency of exosome production and enhance the therapeutic potential of exosome-mediated delivery systems.
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===Usage and Biology===
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===Profile===
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Profile of LAMP2A Signal Peptide
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Name: LAMP2A Signal Peptide <br>
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Base Pairs: 84 bp <br>
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Origins: Derived from the lysosomal-associated membrane protein 2 (LAMP2) gene, commonly found in mammals. <br>
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Properties: The LAMP2A signal peptide facilitates the targeting of proteins to lysosomes, plays a crucial role in the secretion and membrane integration of lysosomal proteins, and is characterized by its hydrophobic nature that promotes membrane interactions.
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===Usage and Biology===
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The LAMP2A signal peptide is commonly used in synthetic biology and genetic engineering to direct proteins to lysosomes. By attaching this signal peptide to the N-terminus of recombinant proteins, researchers can ensure proper localization within the lysosomal compartment, which is essential for the function of lysosomal enzymes and proteins. This application is particularly valuable in therapeutic contexts, such as enzyme replacement therapies for lysosomal storage disorders. <br>
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In mammals, the LAMP2A signal peptide is part of the LAMP2 protein, which is integral to lysosomal membrane dynamics. LAMP2 exists in three isoforms (LAMP2A, LAMP2B, and LAMP2C), with LAMP2A being the primary isoform involved in chaperone-mediated autophagy (CMA). This process allows selective degradation of cytosolic proteins through lysosomal pathways, highlighting the peptide's importance in cellular homeostasis and protein turnover. The LAMP2A signal peptide is critical for recognizing and translocating proteins into lysosomes, contributing to the regulation of lysosomal functions.

Revision as of 18:18, 1 October 2024


LAMP2A signal peptide

The signal peptide of LAMP2A is a short amino acid sequence (typically around 15-30 residues) that serves as a molecular "address" that directs the protein to the secretory pathway.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Profile

Profile of LAMP2A Signal Peptide

Name: LAMP2A Signal Peptide
Base Pairs: 84 bp
Origins: Derived from the lysosomal-associated membrane protein 2 (LAMP2) gene, commonly found in mammals.
Properties: The LAMP2A signal peptide facilitates the targeting of proteins to lysosomes, plays a crucial role in the secretion and membrane integration of lysosomal proteins, and is characterized by its hydrophobic nature that promotes membrane interactions.


Usage and Biology

The LAMP2A signal peptide is commonly used in synthetic biology and genetic engineering to direct proteins to lysosomes. By attaching this signal peptide to the N-terminus of recombinant proteins, researchers can ensure proper localization within the lysosomal compartment, which is essential for the function of lysosomal enzymes and proteins. This application is particularly valuable in therapeutic contexts, such as enzyme replacement therapies for lysosomal storage disorders.
In mammals, the LAMP2A signal peptide is part of the LAMP2 protein, which is integral to lysosomal membrane dynamics. LAMP2 exists in three isoforms (LAMP2A, LAMP2B, and LAMP2C), with LAMP2A being the primary isoform involved in chaperone-mediated autophagy (CMA). This process allows selective degradation of cytosolic proteins through lysosomal pathways, highlighting the peptide's importance in cellular homeostasis and protein turnover. The LAMP2A signal peptide is critical for recognizing and translocating proteins into lysosomes, contributing to the regulation of lysosomal functions.