Difference between revisions of "Part:BBa K247004:Experience"

(User Reviews)
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The Chiba iGEM team tested this part in several conditions.  
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The Chiba iGEM team tested this part in several conditions.
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'''Experiment Condition'''
 
'''Experiment Condition'''
  

Revision as of 03:54, 22 October 2009

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K247004

User Reviews

UNIQ466b303285a37b14-partinfo-00000000-QINU UNIQ466b303285a37b14-partinfo-00000001-QINU

This part properly acted as RFP reporter.

Excitation peak: 584 nm, Emission peak: 607 nm


The Chiba iGEM team tested this part in several conditions.

Experiment Condition

  • AHL concentration of medium : 100 nM
  • Time when we observed mRFP fluorescence : 24 h (since we permeated AHL to this RFP)
  • medium : 1(left) or 5%(right) agar and 10 mL of LB-Amp, Cm medium
Fig. 1 RFP Fluorescent. RFP expression is induced by AHL (100 nM). Observed under UV irradiation.