Difference between revisions of "Part:BBa K193000"
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Backbone of BBa_K193000 is pSB6A1, but Twins are on pSB1 system. | Backbone of BBa_K193000 is pSB6A1, but Twins are on pSB1 system. | ||
[[Image:Tokyo_tech_PL-GFP.jpg]] | [[Image:Tokyo_tech_PL-GFP.jpg]] | ||
| − | + | For detail, see http://2009.igem.org/Team:Tokyo_Tech/Consortium#Result | |
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
===Usage and Biology=== | ===Usage and Biology=== | ||
Revision as of 03:50, 22 October 2009
GFP reporter regulated by CI.
Promoter lambda - cI regulated - (R0051) with GFP (E0040) as a reporter. This part is used to transform E.coli strain N99,which express cI constitutively, and N4830,which express temperature-sensitive cI constitutively.
BBa_S03335 and BBa_J69501 are not available via the Registry distribution.
Backbone of BBa_K193000 is pSB6A1, but Twins are on pSB1 system.
For detail, see http://2009.igem.org/Team:Tokyo_Tech/Consortium#Result
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 719