Difference between revisions of "Part:BBa K227012:Design"

(→‎Design Notes)
(→‎Design Notes)
 
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===Design Notes===
 
===Design Notes===
 
ompR(sph) is codon-optimized for use in R. sphaeroides.
 
ompR(sph) is codon-optimized for use in R. sphaeroides.
 +
A search of R. sphaeroides revealed no EnvZ kinase homologues.[http://2009.igem.org/Team:Wash_U]
 +
When coupled with an introduced system containing an EnvZ kinase, this part would act as an appropriate reporter.
  
 
===Source===
 
===Source===

Latest revision as of 03:43, 22 October 2009

RBS34+OmpR(sphaeroides)+Term+ompC+pucBA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 587
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 629
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

ompR(sph) is codon-optimized for use in R. sphaeroides. A search of R. sphaeroides revealed no EnvZ kinase homologues.[http://2009.igem.org/Team:Wash_U] When coupled with an introduced system containing an EnvZ kinase, this part would act as an appropriate reporter.

Source

composite part, synthesized in two fragments (RBS-Term, ompC+pucBA) from Geneart. Sequences from registry parts.

References