Difference between revisions of "Part:BBa K5401002:Design"

 
 
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<partinfo>BBa_K5401002 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K5401002 SequenceAndFeatures</partinfo>
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===Design Notes===
 
===Design Notes===
 
The design of the plasmid contains a medium-copy number plasmid backbone (BBa_J428341), T7 Promoter (BBa_K3457003), RBS (BBa_J428032), eCFP(BBa_E0020) and T7Terminator (BBa_J428091).
 
The design of the plasmid contains a medium-copy number plasmid backbone (BBa_J428341), T7 Promoter (BBa_K3457003), RBS (BBa_J428032), eCFP(BBa_E0020) and T7Terminator (BBa_J428091).
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===Source===
 
===Source===
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Molecular cloning using ligation; sequence of individual parts were derived from iGEM Registry.
 
Molecular cloning using ligation; sequence of individual parts were derived from iGEM Registry.
  
 
===References===
 
===References===

Latest revision as of 15:45, 1 October 2024


pBRR322_T7Promoter_eCFP


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 2948
    Illegal suffix found in sequence at 16
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2948
    Illegal SpeI site found at 17
    Illegal PstI site found at 31
    Illegal NotI site found at 24
    Illegal NotI site found at 2954
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2948
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 2948
    Illegal suffix found in sequence at 17
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 2948
    Illegal XbaI site found at 2963
    Illegal SpeI site found at 17
    Illegal PstI site found at 31
    Illegal NgoMIV site found at 1390
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 2188

Design Notes

The design of the plasmid contains a medium-copy number plasmid backbone (BBa_J428341), T7 Promoter (BBa_K3457003), RBS (BBa_J428032), eCFP(BBa_E0020) and T7Terminator (BBa_J428091).

Source

Molecular cloning using ligation; sequence of individual parts were derived from iGEM Registry.

References