Difference between revisions of "Part:BBa K5117042"
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This part serves as transcriptional unit composed of: | This part serves as transcriptional unit composed of: | ||
| − | + | <ul> | |
| + | <li>promoter P<i>cotYZ</i> of <i>Bacillus subtilis</i> (BBa_K5117021)</li> | ||
| − | + | <li>ribosome binding site of <i>Bacillus subtilis</i> (BBa_K5117000)</li> | |
| − | + | <li><i>bglB</i> gene of <i>Paenibacillus polymyxa</i> encoding a beta-glucosidase (EC 3.2.1.21)</li> | |
addition of a long flexible linker (L2) downstream of the coding sequence encoding the amino acids (GGGGS)<sub>4</sub> (BBa_K5117030), | addition of a long flexible linker (L2) downstream of the coding sequence encoding the amino acids (GGGGS)<sub>4</sub> (BBa_K5117030), | ||
| − | + | <li><i>cotY</i> gene of <i>Bacillus subtilis</i> (BBa_K5117022)</li> | |
| − | + | <li>bidirectional terminator B0014 (BBa_B0014)</li> | |
| + | </ul> | ||
| + | <b>Biosafety level:</b> S1 | ||
| + | |||
| + | <b>Target organism:</b> <i>Bacillus subtilis</i> | ||
| + | |||
| + | <b>Main purpose of use:</b> Immobilization of PpBglB on the spore crust of <i>B. subtilis</i> (spore surface display) | ||
| + | |||
| + | <b>Application:</b> Degradation of cellobiose | ||
| − | |||
| − | |||
| − | |||
<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
<partinfo>BBa_K5117042 SequenceAndFeatures</partinfo> | <partinfo>BBa_K5117042 SequenceAndFeatures</partinfo> | ||
Revision as of 15:30, 1 October 2024
PcotYZ-BsRBS-PpBglB-L2-CotY-B0014
This part serves as transcriptional unit composed of:
- promoter PcotYZ of Bacillus subtilis (BBa_K5117021)
- ribosome binding site of Bacillus subtilis (BBa_K5117000)
- bglB gene of Paenibacillus polymyxa encoding a beta-glucosidase (EC 3.2.1.21)
- cotY gene of Bacillus subtilis (BBa_K5117022)
- bidirectional terminator B0014 (BBa_B0014)
addition of a long flexible linker (L2) downstream of the coding sequence encoding the amino acids (GGGGS)4 (BBa_K5117030),
Biosafety level: S1
Target organism: Bacillus subtilis
Main purpose of use: Immobilization of PpBglB on the spore crust of B. subtilis (spore surface display)
Application: Degradation of cellobiose
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1627
- 1000COMPATIBLE WITH RFC[1000]