Difference between revisions of "Part:BBa K257021:Experience"

(Applications of BBa_K257021)
(Applications of BBa_K257021)
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'''Conclusion :'''
 
'''Conclusion :'''
 
<br>
 
<br>
In presence of glucose pLac is repressed and then in absence of TetR the pTet promoter is turned on and activates the transcription of mRFP. Accordingly to the construction the fluorescence is higher in our construction in presence of glucose than in the control.
+
In presence of glucose pLac is repressed and then in absence of TetR the pTet promoter is turned on and activates the transcription of mRFP. Accordingly to the construction more bacteria with our construction are fluorescent in presence of glucose than in the control.
  
 
===User Reviews===
 
===User Reviews===

Revision as of 03:31, 22 October 2009

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K257021


PtetRFP trans.jpg
x1000 observation on white light of E. coli with the pLac-TetR::pTet-mRFP construction

PtetRFP fluo.jpg
x1000 observation after fluorescence excitation of E. coli with the pLac-TetR::pTet-mRFP construction

Ptet RFP Glucose trans1.jpg
x1000 observation on white light of E. coli with the pLac-TetR::pTet-mRFP construction in presence of 1% glucose


Ptet RFP Glucose fluo1.jpg
x1000 observation after fluorescence excitation of E. coli with the pLac-TetR::pTet-mRFP construction in presence of 1% glucose


Conclusion :
In presence of glucose pLac is repressed and then in absence of TetR the pTet promoter is turned on and activates the transcription of mRFP. Accordingly to the construction more bacteria with our construction are fluorescent in presence of glucose than in the control.

User Reviews

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