Difference between revisions of "Part:BBa K5323892:Design"

 
Line 7: Line 7:
  
 
===Design Notes===
 
===Design Notes===
−
In order to enable the normal expression of this gene in <i>Acidithiobacillus ferrooxidans</i>, we performed codon optimization.
+
A component that determines whether a downstream gene is expressed or not by a gold detector.
  
  

Revision as of 15:14, 1 October 2024


Ptac-RBS-golS-Pgol-RBS-cI-TT-PR


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1635
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 3721
    Illegal AgeI site found at 1969
    Illegal AgeI site found at 2318
    Illegal AgeI site found at 3256
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

A component that determines whether a downstream gene is expressed or not by a gold detector.


Source

A composite part assembled by RBS(BBa_J34801), golS (BBa_K4468005), Pgol(BBa_K4468001), cI (BBa_R1051), Ptac(BBa_K180000), TT(BBa_B0015) and PR(BBa_K2229450).


References