Difference between revisions of "Part:BBa K212005"
 
Line 5: Line 5:
  
 
===Usage and Biology===
 
===Usage and Biology===
−
This chimeric protein is composed of a trg domain joined to an EnvZ histidine kinase domain.  
+
TrgEnvZ is an archetypal two-component regulatory system that has been studied by Dr. Masayori Inouye since the early 1990s. It constitutes a chimeric protein composed of the extracellular and transmembrane domains of chemoreceptor Trg and the kinase domain of osmosensor EnvZ. Trg is activated by periplasmic binding proteins, which in turn transduces the message to EnvZ to phosphorylate OmpR, which binds to the OmpC promoter, driving transcription of whatever gene is under it.
 +
 
 +
[[Image: trgenvz.png]]
 +
 
 +
''Adapted from Ref. 1 Figure 1''
  
 
<!-- -->
 
<!-- -->

Latest revision as of 03:30, 22 October 2009

TrgEnvZ

transduces RBP signal to OmpC promoter

Usage and Biology

TrgEnvZ is an archetypal two-component regulatory system that has been studied by Dr. Masayori Inouye since the early 1990s. It constitutes a chimeric protein composed of the extracellular and transmembrane domains of chemoreceptor Trg and the kinase domain of osmosensor EnvZ. Trg is activated by periplasmic binding proteins, which in turn transduces the message to EnvZ to phosphorylate OmpR, which binds to the OmpC promoter, driving transcription of whatever gene is under it.

Trgenvz.png

Adapted from Ref. 1 Figure 1

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 348
  • 1000
    COMPATIBLE WITH RFC[1000]