Difference between revisions of "Part:BBa K257021:Experience"
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| − | [[Image:PtetRFP_trans.jpg| | + | [[Image:PtetRFP_trans.jpg|360px]] |
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| − | x1000 observation on white light of E. coli with the pTet-mRFP construction | + | x1000 observation on white light of E. coli with the pLac-TetR::pTet-mRFP construction |
| − | [[Image:PtetRFP_fluo.jpg| | + | [[Image:PtetRFP_fluo.jpg|360px]] |
<br> | <br> | ||
| − | x1000 observation after fluorescence excitation of E. coli with the pTet-mRFP construction | + | x1000 observation after fluorescence excitation of E. coli with the pLac-TetR::pTet-mRFP construction |
| − | [[Image:Ptet_RFP_Glucose_trans1.jpg| | + | [[Image:Ptet_RFP_Glucose_trans1.jpg|360px]] |
<br> | <br> | ||
| − | x1000 observation on white light of E. coli with the pTet-mRFP construction in presence of 1% glucose | + | x1000 observation on white light of E. coli with the pLac-TetR::pTet-mRFP construction in presence of 1% glucose |
| − | [[Image:Ptet_RFP_Glucose_fluo1.jpg| | + | [[Image:Ptet_RFP_Glucose_fluo1.jpg|360px]] |
<br> | <br> | ||
| − | x1000 observation after fluorescence excitation of E. coli with the pTet-mRFP construction in presence of 1% glucose | + | x1000 observation after fluorescence excitation of E. coli with the pLac-TetR::pTet-mRFP construction in presence of 1% glucose |
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| + | |||
| + | '''Conclusion :''' | ||
| + | <br> | ||
| + | In presence of glucose pLac is repressed and then in absence of TetR the pTet promoter is turned on and activates the transcription of mRFP. Accordingly to the construction the fluorescence is higher in our construction in presence of glucose than in the control. | ||
===User Reviews=== | ===User Reviews=== | ||
Revision as of 03:28, 22 October 2009
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K257021
x1000 observation on white light of E. coli with the pLac-TetR::pTet-mRFP construction
x1000 observation after fluorescence excitation of E. coli with the pLac-TetR::pTet-mRFP construction
x1000 observation on white light of E. coli with the pLac-TetR::pTet-mRFP construction in presence of 1% glucose
x1000 observation after fluorescence excitation of E. coli with the pLac-TetR::pTet-mRFP construction in presence of 1% glucose
Conclusion :
In presence of glucose pLac is repressed and then in absence of TetR the pTet promoter is turned on and activates the transcription of mRFP. Accordingly to the construction the fluorescence is higher in our construction in presence of glucose than in the control.
User Reviews
UNIQ76811ceeb38f6d3f-partinfo-00000000-QINU UNIQ76811ceeb38f6d3f-partinfo-00000001-QINU