Difference between revisions of "Part:BBa K5201001"
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<partinfo>BBa_K5201001 short</partinfo>
 
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Biology
 
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kfiD encodes UDP-glucose-6-dehydrogenase (UGDH) from E. coli K5 strain.
 
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Usage and design
 
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UGDH is endogenously expressed in a low level in E. coli, our recombinant plasmid will overexpress UGDH under an inducible promoter (BBa_R0010). We hope that overexpression of kfiD can increase production of Hyaluronic Acid (HA), by converting UDP-glucose to UDP-glucuronic acid, one of the precursors of HA. HA is widely utilized by the cosmetic industry for its significant water absorption and retention properties. And therefore, we want to further implement it for agricultural use.
 
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===Usage and Biology===-->
 
===Usage and Biology===-->
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===Characterisation of BBa_K5201001: HongKong-UCCKE===
 
===Characterisation of BBa_K5201001: HongKong-UCCKE===
 
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Revision as of 15:05, 1 October 2024

kfiD codons are optimized for overexpression of UDP-glucose-6-dehyrogenase

Characterisation of BBa_K5201001: HongKong-UCCKE

Document

Biology

kfiD encodes UDP-glucose-6-dehydrogenase (UGDH) from E. coli K5 strain.



Usage and design

UGDH is endogenously expressed in a low level in E. coli, our recombinant plasmid will overexpress UGDH under an inducible promoter (BBa_R0010). We hope that overexpression of kfiD can increase production of Hyaluronic Acid, by converting UDP-glucose to UDP-glucuronic acid, one of the precursors of HA. HA is widely utilized by the cosmetic industry for its significant water absorption and retention properties. And therefore, we want to further implement it for agricultural use.