Difference between revisions of "Part:BBa K5492010"
| Line 11: | Line 11: | ||
<!-- --> | <!-- --> | ||
===Experiments=== | ===Experiments=== | ||
| − | ==PCR== | + | ==PCR - optimised dao== |
We have found the following PCR protocol the most optimal for the part: | We have found the following PCR protocol the most optimal for the part: | ||
https://static.igem.wiki/teams/5492/registry/dao-pcr-plan.png | https://static.igem.wiki/teams/5492/registry/dao-pcr-plan.png | ||
| − | |||
We added the following substrates in order: | We added the following substrates in order: | ||
| Line 23: | Line 22: | ||
| − | + | We added 6X DNA Loading dye to each PCR tubes. | |
| − | We added | + | |
We loaded the wells of the gels with 12μL of DAO solution in the following order: | We loaded the wells of the gels with 12μL of DAO solution in the following order: | ||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
https://static.igem.wiki/teams/5492/registry/opt-dao-pcr.png | https://static.igem.wiki/teams/5492/registry/opt-dao-pcr.png | ||
| + | |||
| + | 1.D31O 2.D32O 3.D33O 4.D34O 5.Ladder | ||
<partinfo>BBa_K5492010 SequenceAndFeatures</partinfo> | <partinfo>BBa_K5492010 SequenceAndFeatures</partinfo> | ||
Revision as of 14:45, 1 October 2024
Twist adapter forward primer
Forward primer sequence of the adapter sequence utilised by Twist Bioscience. Utilised in PCR reactions for part BBa_K5492202 and BBa_K5492203.
Experiments
PCR - optimised dao
We have found the following PCR protocol the most optimal for the part:
We added the following substrates in order:
We added 6X DNA Loading dye to each PCR tubes. We loaded the wells of the gels with 12μL of DAO solution in the following order:
1.D31O 2.D32O 3.D33O 4.D34O 5.Ladder
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]