Difference between revisions of "Part:BBa K5143006"
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<img src="https://static.igem.wiki/teams/5143/bba-k5143006-fwyellow-gene.png" width="400" alt="fwYellow"> | <img src="https://static.igem.wiki/teams/5143/bba-k5143006-fwyellow-gene.png" width="400" alt="fwYellow"> | ||
| − | <figcaption>Figure 1: fwYellow gene</figcaption> | + | <figcaption><i><u>Figure 1:</u> fwYellow gene</i></figcaption> |
</figure> | </figure> | ||
<figure> | <figure> | ||
<img src="https://static.igem.wiki/teams/5143/bba-k5143006-yellow-cellulose.png" width="200" alt="yellow cellulose"> | <img src="https://static.igem.wiki/teams/5143/bba-k5143006-yellow-cellulose.png" width="200" alt="yellow cellulose"> | ||
| − | <figcaption>Figure 2: Representation of fwYellow binded to bacterial cellulose</figcaption> | + | <figcaption><i><u>Figure 2:</u> Representation of fwYellow binded to bacterial cellulose</i></figcaption> |
</figure> | </figure> | ||
</div> | </div> | ||
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<h1>Construction</h1> | <h1>Construction</h1> | ||
<p> | <p> | ||
The fwYellow gene was synthesized and its nucleotide sequence optimized for synthesis and expression in <i>Saccharomyces cerevisiae</i>. We used the fwYellow fused to a yeast secretion signal, the alpha-factor (<a href="https://parts.igem.org/Part:BBa_K5143009">BBa_K5143009</a>) in 5' and to a cellulose binding domain (<a href="https://parts.igem.org/Part:BBa_K5143007">BBa_K5143007</a>) in 3'. See the composite part here : <a href="https://parts.igem.org/Part:BBa_K5143023">BBa_K5143023</a> | The fwYellow gene was synthesized and its nucleotide sequence optimized for synthesis and expression in <i>Saccharomyces cerevisiae</i>. We used the fwYellow fused to a yeast secretion signal, the alpha-factor (<a href="https://parts.igem.org/Part:BBa_K5143009">BBa_K5143009</a>) in 5' and to a cellulose binding domain (<a href="https://parts.igem.org/Part:BBa_K5143007">BBa_K5143007</a>) in 3'. See the composite part here : <a href="https://parts.igem.org/Part:BBa_K5143023">BBa_K5143023</a> | ||
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<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
===Usage and Biology=== | ===Usage and Biology=== | ||
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<h1>Sequence and Features</h1> | <h1>Sequence and Features</h1> | ||
<partinfo>BBa_K5143006 SequenceAndFeatures</partinfo> | <partinfo>BBa_K5143006 SequenceAndFeatures</partinfo> | ||
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| − | + | <h1>References</h1> | |
| + | <p> | ||
| + | 1. iGEM13_Uppsala<br> | ||
| + | 2. Liljeruhm, J. et al. Engineering a palette of eukaryotic chromoproteins for bacterial synthetic biology. Journal of Biological Engineering 12, 8 (2018). | ||
| + | </p> | ||
<!-- Uncomment this to enable Functional Parameter display | <!-- Uncomment this to enable Functional Parameter display | ||
===Functional Parameters=== | ===Functional Parameters=== | ||
<partinfo>BBa_K5143006 parameters</partinfo> | <partinfo>BBa_K5143006 parameters</partinfo> | ||
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Latest revision as of 14:15, 1 October 2024
fwYellow, chromoprotein optimized for expression in Saccharomyces cerevisiae
Description
fwYellow is a chromoprotein that was synthetized to monitor biological processes, like fluorescent proteins, but without the need to use devices to detect the protein. Chromoproteins absorb visible light so that they are visible in the ambient light unlike fluorescent proteins. fwYellow was first synthetized by the iGEM team Uppsala in 2013 (BBa_K1033910). The excitation wavelength is 520nm and the emission wavelength is 540nm. Here, the fwYellow chromoprotein was transformed into Saccharomyces cerevisiae cocultured with the cellulose-producing bacteria Komagataeibacter rhaeticus in order to make the cellulose yellow. The coloured cellulose, that should also be sticky thanks to a bioglue (BBa_K5143022) would then trap insects that are specifically attracted to yellow.
Construction
The fwYellow gene was synthesized and its nucleotide sequence optimized for synthesis and expression in Saccharomyces cerevisiae. We used the fwYellow fused to a yeast secretion signal, the alpha-factor (BBa_K5143009) in 5' and to a cellulose binding domain (BBa_K5143007) in 3'. See the composite part here : BBa_K5143023
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 427
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 427
- 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 427
- 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 427
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 427
- 1000COMPATIBLE WITH RFC[1000]
References
1. iGEM13_Uppsala
2. Liljeruhm, J. et al. Engineering a palette of eukaryotic chromoproteins for bacterial synthetic biology. Journal of Biological Engineering 12, 8 (2018).