Difference between revisions of "Part:BBa K5291050"

 
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===Usage and Biology===
 
===Usage and Biology===
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In degradation module we constructed pAB1-pS-PEBP-PEase to depolymerize PE microplastics.<br>
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<html><img width = "700" src=" " /></html><br>
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<b>Fig.1 The map of plasmid pAB1-pS-PEBP-GFP.</b><br><br>
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Moreover, PEBP-GFP proteins was successfully expressed in the strains. SDS-PAGE was performed and the following results were obtained.<br>
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<html><img width = "500" src="https://static.igem.wiki/teams/5291/images/result/degradation/sds-page-result-of-pebp-gfp.png" /></html><br>
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<b>Fig.2 The SDS-PAGE result of PEBP-GFP.</b><br><br>
  
 
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Revision as of 14:11, 1 October 2024


PE-binding peptide

Plastic-binding peptides are adsorbed to the plastic surface by hydrogen bonding and other intermolecular forces. Considering that PE-binding peptides（PEBP） has not been extensively studied and we did not find the sequence of PEBP in the paper, we decided to predict PEBP sequence by modeling method. We performed machine learning training; then performed special processing and tried to introduce neural networks. Finally, several predictors were given.


Usage and Biology

In degradation module we constructed pAB1-pS-PEBP-PEase to depolymerize PE microplastics.


Fig.1 The map of plasmid pAB1-pS-PEBP-GFP.

Moreover, PEBP-GFP proteins was successfully expressed in the strains. SDS-PAGE was performed and the following results were obtained.


Fig.2 The SDS-PAGE result of PEBP-GFP.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]