Difference between revisions of "Part:BBa K5117045"

Revision as of 14:03, 1 October 2024

PcotYZ-BsRBS-BhrPET-L2-CotY-B0014

This part serves as transcriptional unit composed of:

- promoter PcotYZ of Bacillus subtilis (BBa_K5117021),

- ribosome binding site of Bacillus subtilis (BBa_K5117000),

- gene of the uncultured bacterium HR29 encoding a polyethylene terephthalate hydrolase (PETase, EC 3.1.1.101), a homologue of the leaf-branch compost cutinase (LCC), codon optimized for Bacillus subtilis (Xi et al. 2021) without any signal peptide,

addition of a long flexible linker (L2) downstream of the coding sequence encoding the amino acids (GGGGS)₄ (BBa_K5117033),

- cotY gene of Bacillus subtilis (BBa_K5117022),

- bidirectional terminator B0014 (BBa_B0014).

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 1063
Illegal AgeI site found at 695
Illegal AgeI site found at 740
1000
COMPATIBLE WITH RFC[1000]

@@ Line 11: / Line 11: @@
 - gene of the uncultured bacterium HR29 encoding a polyethylene terephthalate hydrolase (PETase, EC 3.1.1.101), a homologue of the leaf-branch compost cutinase (LCC), codon optimized for <i>Bacillus subtilis</i> (Xi <i>et al.</i> 2021) without any signal peptide,
-addition of a long flexible linker (L2) downstream of the coding sequence encoding the amino acids (GGGGS)4 (BBa_K5117033),
+addition of a long flexible linker (L2) downstream of the coding sequence encoding the amino acids (GGGGS)<sub>4</sub> (BBa_K5117033),
 - <i>cotY</i> gene of <i>Bacillus subtilis</i> (BBa_K5117022),