Difference between revisions of "Part:BBa K231000"

Revision as of 03:11, 22 October 2009

Metal binding peptide

This part codes for a peptide containing a metal binding motif. De novo design allows this peptide to have high affinity for metals. Affinity derives from Cys-Gly repeats. This peptide encodes two Cys-Gly-Cys-Cys-Gly repeats. Other metals besides arsenic, such as Cd and Hg, should be bound as well. A similar sequence was reported to successfully increase the ability of E. coli to sequester and survive high levels of heavy metals (Pazirandeh, M., Wells, B., Ryan, R. Development of Bacterium-Based Heavy Metal Biosorbents: Enhanced Uptake of Cadmium and Mercury by Excherichia coli Expressing a Metal Binding Motif. Appl Environ Microbiol. 64, 4068-4072 (1998).)

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 __NOTOC__
 <partinfo>BBa_K231000 short</partinfo>
-This part codes for a peptide containing a metal binding motif. De novo design allows this peptide to have high affinity for metals. Affinity derives from Cys-Gly repeats. This peptide encodes two Cys-Gly-Cys-Cys-Gly repeats. Other metals besides arsenic, such as Cd and Hg, should be bound as well.
+This part codes for a peptide containing a metal binding motif. De novo design allows this peptide to have high affinity for metals. Affinity derives from Cys-Gly repeats. This peptide encodes two Cys-Gly-Cys-Cys-Gly repeats. Other metals besides arsenic, such as Cd and Hg, should be bound as well.  A similar sequence was reported to successfully increase the ability of E. coli to sequester and survive high levels of heavy metals (Pazirandeh, M., Wells, B., Ryan, R. Development of Bacterium-Based Heavy Metal Biosorbents: Enhanced Uptake of Cadmium and Mercury by Excherichia coli Expressing a Metal Binding Motif. Appl Environ Microbiol. 64, 4068-4072 (1998).)
 <!-- Add more about the biology of this part here