Difference between revisions of "Part:BBa K5059001"
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This is a composite part that integrates a strong constitutive promoter (BBa_K124002), Kozak sequence (BBa_K165002), Team Cornell's BBA_K5059000 BioBrick that encodes for alpha-amyrin synthase (AAS), and a strong terminator (BBa_K2926005). Implementing these BioBricks together allows for increased expression of AAS, outputting more alpha-amyrin for ursolic acid production.
 
This is a composite part that integrates a strong constitutive promoter (BBa_K124002), Kozak sequence (BBa_K165002), Team Cornell's BBA_K5059000 BioBrick that encodes for alpha-amyrin synthase (AAS), and a strong terminator (BBa_K2926005). Implementing these BioBricks together allows for increased expression of AAS, outputting more alpha-amyrin for ursolic acid production.
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===Sequence and Features===
 
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<partinfo>BBa_K5059000 SequenceAndFeatures</partinfo>
 
  
 
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Revision as of 13:42, 1 October 2024


TDH3-AAS-TPS1

This is a composite part that integrates a strong constitutive promoter (BBa_K124002), Kozak sequence (BBa_K165002), Team Cornell's BBA_K5059000 BioBrick that encodes for alpha-amyrin synthase (AAS), and a strong terminator (BBa_K2926005). Implementing these BioBricks together allows for increased expression of AAS, outputting more alpha-amyrin for ursolic acid production.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Figure 1: Metabolic Pathway for Producing Ursolic Acid in Yeast

Within this pathway, TDH3-AAS-TPS1 is the first enzyme required to catalyze ursolic acid production. Specifically, it catalyzes the conversion of 2,3-oxidosqualene to alpha-amyrin, which is the direct precursor for ursolic acid through the action of Cytochrome P450, which is activated via Cytochrome P450 reductase donating an electron from NADPH to it.

Functionality

Catalytic Efficiency

Substrate Product Km (µM) kcat (min^-1) kcat/Km (min^-1/µM)
1. 2,3-oxidosqualene alpha-amyrin 50.07 43.4 0.87
2. alpha-amyrin Ursolic Acid 24.5 35 1.43
This data comes from Dr. Yu et al.[3].