Difference between revisions of "Part:BBa K5302013:Design"

 
Line 13: Line 13:
 
===Source===
 
===Source===
  
−
This work is derived from pBBR1MCS-mCherry and pUC19-Lpp-OmpA, and it has undergone codon optimization.
+
Lpp: Hunman
 +
OmpA: Escherichia coli (strain K12)
 +
mCherry: Mushroom coral
  
 
===References===
 
===References===

Revision as of 13:35, 1 October 2024


pBBR-LppOmpA-mCherry


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 3631
    Illegal EcoRI site found at 3679
    Illegal EcoRI site found at 3952
    Illegal PstI site found at 2016
    Illegal PstI site found at 3940
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 3631
    Illegal EcoRI site found at 3679
    Illegal EcoRI site found at 3952
    Illegal PstI site found at 2016
    Illegal PstI site found at 3940
    Illegal NotI site found at 1057
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 3631
    Illegal EcoRI site found at 3679
    Illegal EcoRI site found at 3952
    Illegal BglII site found at 1803
    Illegal BamHI site found at 3583
    Illegal XhoI site found at 4297
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 3631
    Illegal EcoRI site found at 3679
    Illegal EcoRI site found at 3952
    Illegal PstI site found at 2016
    Illegal PstI site found at 3940
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 3631
    Illegal EcoRI site found at 3679
    Illegal EcoRI site found at 3952
    Illegal PstI site found at 2016
    Illegal PstI site found at 3940
    Illegal NgoMIV site found at 2467
    Illegal NgoMIV site found at 2750
    Illegal NgoMIV site found at 3538
    Illegal NgoMIV site found at 4723
    Illegal AgeI site found at 4158
    Illegal AgeI site found at 4563
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1379
    Illegal SapI.rc site found at 2316
    Illegal SapI.rc site found at 2526


Design Notes

Please be advised that all listed genes have undergone codon optimization for our chassis strain, Escherichia coli Nissle 1917 (EcN). For those considering alternative chassis organisms, it is recommended to reassess the necessity of codon optimization.


Source

Lpp: Hunman OmpA: Escherichia coli (strain K12) mCherry: Mushroom coral

References