Difference between revisions of "Part:BBa K5322031:Design"
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===Source=== | ===Source=== | ||
| − | + | Components from BBa_J23119, BBa_B0034, BBa_K5322034, BBa_K731721. | |
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===References=== | ===References=== | ||
Revision as of 13:30, 1 October 2024
Programmed Cell Death 1 Ligand 1 Functional Domain [Mus musculus] Expression System
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 377
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal NheI site found at 519
Illegal PstI site found at 377 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 277
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 377
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 377
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Codon optimization was performed according to the E. coli codon preference table. PD-L1 was truncated, retaining the 1st to 150aa of the original PD-L1 290aa.
Source
Components from BBa_J23119, BBa_B0034, BBa_K5322034, BBa_K731721.