Difference between revisions of "Part:BBa K5322031:Design"

 
(→‎Design Notes)
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===Design Notes===
 
===Design Notes===
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Codon optimization was performed according to the <i>E. coli</i> codon preference table. PD-L1 was truncated, retaining the 1st to 150aa of the original PD-L1 290aa.
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===Source===
 
===Source===

Revision as of 13:29, 1 October 2024


Programmed Cell Death 1 Ligand 1 Functional Domain [Mus musculus] Expression System


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 377
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 519
    Illegal PstI site found at 377
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 277
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 377
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 377
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Codon optimization was performed according to the E. coli codon preference table. PD-L1 was truncated, retaining the 1st to 150aa of the original PD-L1 290aa.

Source

1234

References