Difference between revisions of "Part:BBa K218005:Design"

(→‎References)
(→‎Source)
 
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===Source===
 
===Source===
  
−
GenBank Accession# L26221 LuxO Protein ID# AAD12736.1 LuxU Protein ID# AAD12737.2
+
LuxOU - GenBank Accession# L26221 LuxO Protein ID# AAD12736.1; LuxU Protein ID# AAD12737.2
  
−
The source of the B0015 terminators and the R0040 promoter was the Registry of Standard Biological Parts.
+
B0015, R0040 - Registry of Standard Biological Parts.
  
 
===References===
 
===References===
 
Jeremy A. Freeman and Bonnie L. Bassler. Sequence and function of LuxU: a two-component phosphorelay protein that regulates quorum sensing in Vibrio harveyi. 1999. Journal of Bacteriology. Feb 1999, p. 899-906.
 
Jeremy A. Freeman and Bonnie L. Bassler. Sequence and function of LuxU: a two-component phosphorelay protein that regulates quorum sensing in Vibrio harveyi. 1999. Journal of Bacteriology. Feb 1999, p. 899-906.

Latest revision as of 03:03, 22 October 2009

LuxOU generator with terminator upstream


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1053
    Illegal SapI.rc site found at 1015


Design Notes

No RBS was cloned into this intermediate because the LuxOU operons already has an internal RBS. Although there is an internal terminator as well, we have constructed the strong terminator B0015 after this coding sequence in order to provide further insulation of the circuit. The B0015 terminator was cloned upstream of the LuxOU generator because this intermediate will allow for subsequent cloning and insulation of LuxPQ upstream of this terminator.

Source

LuxOU - GenBank Accession# L26221 LuxO Protein ID# AAD12736.1; LuxU Protein ID# AAD12737.2

B0015, R0040 - Registry of Standard Biological Parts.

References

Jeremy A. Freeman and Bonnie L. Bassler. Sequence and function of LuxU: a two-component phosphorelay protein that regulates quorum sensing in Vibrio harveyi. 1999. Journal of Bacteriology. Feb 1999, p. 899-906.