Difference between revisions of "Part:BBa K5322013:Design"
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===Source=== | ===Source=== | ||
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| − | The team obtains the SOD-1 sequence from <a href="https://parts.igem.org/Part:BBa_K2215003" | + | The team obtains the SOD-1 sequence from <a href="https://parts.igem.org/Part:BBa_K2215003">BBa_K2215003</a>. |
</html> | </html> | ||
===References=== | ===References=== | ||
Latest revision as of 12:51, 1 October 2024
pET29a-J23119-RBS-SoxR-T7-pSoxS-RBS-(SOD-1)-T7.
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal NheI site found at 552
Illegal NheI site found at 1157 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
To ensure the successful expression of SOD-1 in Escherichia coli, we performed codon optimization of the SOD-1 sequence.
Source
The team obtains the SOD-1 sequence from BBa_K2215003.