Difference between revisions of "Part:BBa K231001"

Revision as of 03:00, 22 October 2009

Phytochelatin Synthase, HA-tag

Codes for phytochelatin synthase (PCS) which catalyzes the conversion of glutathione (GSH) to phytochelatin (PC). HA Tag appended after a TG scar/linker to enhance protein detection ability. The precursors for PC are naturally present in E. coli and have been shown to be upregulated in the presence of PCS and heavy metals (Li, Dhankher, Carriera. 2004). PC is a cysteine rich molecule capable of binding heavy metals. The PCS gene is endogenous to A. thaliana.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Unknown
12
INCOMPATIBLE WITH RFC[12]
Unknown
21
INCOMPATIBLE WITH RFC[21]
Illegal EcoRI site found at 1
23
INCOMPATIBLE WITH RFC[23]
Illegal EcoRI site found at 1
Illegal PstI site found at 100
Illegal PstI site found at 1363
Illegal PstI site found at 1396
Illegal PstI site found at 1507
25
INCOMPATIBLE WITH RFC[25]
Illegal EcoRI site found at 1
Illegal PstI site found at 100
Illegal PstI site found at 1363
Illegal PstI site found at 1396
Illegal PstI site found at 1507
Illegal NgoMIV site found at 1449
1000
COMPATIBLE WITH RFC[1000]

Revision as of 02:59, 22 October 2009 (view source) ThaddeusWebb (Talk \| contribs) ← Older edit		Revision as of 03:00, 22 October 2009 (view source) ThaddeusWebb (Talk \| contribs) Newer edit →
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	<partinfo>BBa_K231001 short</partinfo>		<partinfo>BBa_K231001 short</partinfo>

−	Codes for phytochelatin synthase (PCS) which catalyzes the conversion of glutathione (GSH) to phytochelatin (PC). HA Tag appended after a TG scar/linker to enhance protein detection ability. The precursors for PC are naturally present in E. coli and have been shown to be upregulated in the presence of PCS and heavy metals (Li, Dhankher, Carriera. 2004). PC is a cysteine rich molecule capable of binding heavy metals. The PCS gene is endogenous to A. thaliana	+	Codes for phytochelatin synthase (PCS) which catalyzes the conversion of glutathione (GSH) to phytochelatin (PC). HA Tag appended after a TG scar/linker to enhance protein detection ability. The precursors for PC are naturally present in E. coli and have been shown to be upregulated in the presence of PCS and heavy metals (Li, Dhankher, Carriera. 2004). PC is a cysteine rich molecule capable of binding heavy metals. The PCS gene is endogenous to A. thaliana.