Difference between revisions of "Part:BBa K5322013"
(→Construction of the plasmid) |
Jiangjiang (Talk | contribs) (→Construction of the plasmid) |
||
| Line 43: | Line 43: | ||
</style> | </style> | ||
<div class="center-img"> | <div class="center-img"> | ||
| − | <img src="https://static.igem.wiki/teams/5322/ | + | <img src="https://static.igem.wiki/teams/5322/wet-lab/p-soxr-t-psoxs-rbs-sod-t-map.png" alt="pET29a-J23119-RBS-SoxR-T7-pSoxS-RBS-(SOD-1)-T7" width="300"> |
<p align="center"><b>Figure 2-1</b> Plasmid pET29a-J23119-RBS-SoxR-T7-pSoxS-RBS-(SOD-1)-T7</p> | <p align="center"><b>Figure 2-1</b> Plasmid pET29a-J23119-RBS-SoxR-T7-pSoxS-RBS-(SOD-1)-T7</p> | ||
</div> | </div> | ||
Revision as of 12:48, 1 October 2024
pET29a-J23119-RBS-SoxR-T7-pSoxS-RBS-(SOD-1)-T7.
Contents
Usage and Biology
Plasmid pET29a-J23119-RBS-SoxR-T7-pSoxS-RBS-(SOD-1)-T7,the pET29a vector is used for high-level expression in E. coli. The expression of SOD-1 is controlled by the NO-responsive promoter SoxR/SoxS. The ribosome binding site (RBS) ensures efficient translation of mRNA, while the T7 terminator provides a clean and efficient end for transcription. This system is designed to express SOD only in the intestinal inflammation site and exert its antioxidant effect.
Structural design
Our team designed the gene circuit shown in the figure below: pET29a-J23119-RBS-SoxR-T7-pSoxS-RBS-(SOD-1)-T7.
Figure 1-1 gene circuit pET29a-J23119-RBS-SoxR-T7-pSoxS-RBS-(SOD-1)-T7
Construction of the plasmid
Our team designed the plasmid pET29a-J23119-RBS-SoxR-T7-pSoxS-RBS-(SOD-1)-T7, and the plasmid map is illustrated below.
Figure 2-1 Plasmid pET29a-J23119-RBS-SoxR-T7-pSoxS-RBS-(SOD-1)-T7
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal NheI site found at 552
Illegal NheI site found at 1157 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]