Difference between revisions of "Part:BBa K5382123"

Latest revision as of 12:22, 1 October 2024

sfGFP-superfolder green fluorescent protein

The sfGFP, or Superfolder GFP, is a genetically engineered variant of green fluorescent protein (GFP).It has rapid maturation properties and maintains a good folding state even when fused with other poorly folded peptides. With a molecular weight of approximately 26.8 kDa, sfGFP is a weak dimer with advantages including improved tolerance to circular arrangement, greater tolerance to chemical denaturation, and improved folding kinetics.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K5382123 short</partinfo>
-The sfGFP, or Superfolder GFP, is a genetically engineered variant of green fluorescent protein (GFP).
+The sfGFP, or Superfolder GFP, is a genetically engineered variant of green fluorescent protein (GFP).It has rapid maturation properties and maintains a good folding state even when fused with other poorly folded peptides. With a molecular weight of approximately 26.8 kDa, sfGFP is a weak dimer with advantages including improved tolerance to circular arrangement, greater tolerance to chemical denaturation, and improved folding kinetics.
-The composite part formed by the series combination of the three is mainly through the high affinity of CL7 and Im7, and through incubation, CL7-Linker-SFGFP is combined with Inak-linker-Im7(another composite part registered by us), thus anchoring the integration system on the membrane surface. Finally, the Inak-linker-Im7-CL7-linker-sfGFP system was displayed on the surface of the membrane, so that sfGFP protein could play a role. Here, the role of sfGFP protein was tested by double fluorescence verification (for details, see the detail consideration part in the experimental design) whether the system was successfully displayed on the membrane surface. It should be noted that the system we designed uses the high affinity of CL7 and Im7, so some components can be flexibly replaced according to needs, and are suitable for different use scenarios according to different needs. For example, according to different cells to replace the ice nucleated protein, according to different targeting targets to replace sfGFP with different single-chain antibodies, nano antibodies and so on.
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