Difference between revisions of "Part:BBa K5382124:Design"
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− | CL7, an engineered variant of colicin CE7, is a protein tag obtained by engineering CE7 to remove its DNA-binding and catalytic activity, preserving its high affinity binding ability to the corresponding inhibitory protein Im7 | + | CL7, an engineered variant of colicin CE7, is a protein tag obtained by engineering CE7 to remove its DNA-binding and catalytic activity, preserving its high affinity binding ability to the corresponding inhibitory protein Im7. |
Revision as of 12:10, 1 October 2024
CL7-protein tag
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 133
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 133
- 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 133
Illegal BglII site found at 81 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 133
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 133
Illegal AgeI site found at 31
Illegal AgeI site found at 45 - 1000COMPATIBLE WITH RFC[1000]
Source
CL7, an engineered variant of colicin CE7, is a protein tag obtained by engineering CE7 to remove its DNA-binding and catalytic activity, preserving its high affinity binding ability to the corresponding inhibitory protein Im7.