Difference between revisions of "Part:BBa K5034212:Design"
 
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===Design Notes===
 
===Design Notes===
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We synthesized the fragment using a chemical synthesis method, with the aim of expressing the protein in Shewanella and regulating cellular phosphorus metabolism and electron transfer.
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We synthesized the fragment using a chemical synthesis method, with the aim of expressing the protein in ''S. oneidensis'' and regulating cellular phosphorus metabolism and electron transfer.
  
  

Latest revision as of 11:44, 1 October 2024


PolyP + AMP --> ADP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We synthesized the fragment using a chemical synthesis method, with the aim of expressing the protein in S. oneidensis and regulating cellular phosphorus metabolism and electron transfer.


Source

Phosphate-AMP phosphotransferase(PAP) from Acinetobacter johnsonii. NCBI reference sequence: AB092983.1

References

Itoh, H., & Shiba, T. (2004). Polyphosphate synthetic activity of polyphosphate:AMP phosphotransferase in Acinetobacter johnsonii 210A. Journal of Bacteriology, 186(15), 5178-5181. doi:10.1128/jb.186.15.5178-5181.2004