Difference between revisions of "Part:BBa K194002"

Revision as of 02:48, 22 October 2009

Destabilized GFP for yeast

This variant of biobrick BBa_K194001 and improvement of BBa_E0040 has been destabilized by the fusion of biobrick BBa_K194000 and BBa_K194001. In the article by Mateus and Avery: "Destabilized green Fluorescent protein for monitoring dynamic changes in yeast gene expression with flow cytometry" they show that addition of these 178 carboxyl-terminal amino acid residues of the Cln2 PEST signal, changes the half-life of a GFP from 7h and down to 30 minutes.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 644

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K194002 short</partinfo>
-This variant of biobrick BBa_K194001 has been destabilized by fusing biobrick BBa_K194000.
+This variant of biobrick BBa_K194001 and improvement of <html><a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_E0040" target="_blank">BBa_E0040</a></html> has been destabilized by the fusion of biobrick BBa_K194000 and BBa_K194001.
 In the article by Mateus and Avery: "Destabilized green Fluorescent protein for monitoring dynamic changes in yeast gene expression with flow cytometry" they show that addition of these 178 carboxyl-terminal amino acid residues of the Cln2 PEST signal, changes the half-life of a GFP from 7h and down to 30 minutes.