Difference between revisions of "Part:BBa K243002"

Revision as of 02:48, 22 October 2009

DsbA signal sequence (enables periplasm export)

DsbA encodes a periplasmic disulfide bond isomerase. Lokalized in the periplasm, it catalyzes the forming of disulfide bonds and therefore enables the correct folding of proteins in this compartment.

Usage and Biology

The signal sequence of the DsbA protein linked to the coding sequence of another protein enables export of the latter into the periplasm. The accumulation of the expressed protein in the periplasm can be used for the purification of the protein and to prevent interaction with cytoplasmic components.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 9: / Line 9: @@
 ===Usage and Biology===
-The signal sequence of DsbA linked to the coding sequence of e.g.a protein, enables the export of the protein into the periplasm. The accumulation of the expressed protein in the periplasm can be used for the purification of the protein.
+The signal sequence of the DsbA protein linked to the coding sequence of another protein enables export of the latter into the periplasm. The accumulation of the expressed protein in the periplasm can be used for the purification of the protein and to prevent interaction with cytoplasmic components.
 [[Image:Freiburg 09 DsbA grafik.jpg|550x550px]]<br>