Difference between revisions of "Part:BBa K5302033"
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This work is derived from pBBR-OmpA-VEGFR1D2 and pUC19-VEGFR-masking#18, and it has undergone codon optimization. This composite part combines OmpA(21.4kda) and VEGFR1D2(approximately 12kda), and it adds a fragment as a mask. We succeeded in transferring this plasmid into Escherichia coli Nissle 1917 and let it express VEGFR1D2. The plasmid uses lac promotor and has kanamycin resistence.
 
This work is derived from pBBR-OmpA-VEGFR1D2 and pUC19-VEGFR-masking#18, and it has undergone codon optimization. This composite part combines OmpA(21.4kda) and VEGFR1D2(approximately 12kda), and it adds a fragment as a mask. We succeeded in transferring this plasmid into Escherichia coli Nissle 1917 and let it express VEGFR1D2. The plasmid uses lac promotor and has kanamycin resistence.
  
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    <img src="https://static.igem.wiki/teams/5302/images/part-registry-p-o-vegfr1d2-l229-1.png"
 
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            <b>Figure 1. </b> Colony PCR results of pBBR-OmpA-VEGFR1D2-l2masking#29
 
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 10:33, 1 October 2024


OmpA-VEGFR1D2-l2masking#18

This work is derived from pBBR-OmpA-VEGFR1D2 and pUC19-VEGFR-masking#18, and it has undergone codon optimization. This composite part combines OmpA(21.4kda) and VEGFR1D2(approximately 12kda), and it adds a fragment as a mask. We succeeded in transferring this plasmid into Escherichia coli Nissle 1917 and let it express VEGFR1D2. The plasmid uses lac promotor and has kanamycin resistence.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2376
    Illegal XbaI site found at 1296
    Illegal PstI site found at 123
    Illegal PstI site found at 1284
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2376
    Illegal PstI site found at 123
    Illegal PstI site found at 1284
    Illegal NotI site found at 5308
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2376
    Illegal BglII site found at 6054
    Illegal BamHI site found at 1302
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2376
    Illegal XbaI site found at 1296
    Illegal PstI site found at 123
    Illegal PstI site found at 1284
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2376
    Illegal XbaI site found at 1296
    Illegal PstI site found at 123
    Illegal PstI site found at 1284
    Illegal NgoMIV site found at 574
    Illegal NgoMIV site found at 857
    Illegal NgoMIV site found at 3034
    Illegal AgeI site found at 1955
    Illegal AgeI site found at 2874
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 5630
    Illegal SapI.rc site found at 423
    Illegal SapI.rc site found at 633