Difference between revisions of "Part:BBa K5302025"

 
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This work is derived from pUC19-8IIU and pBBR1MCS-INP-mCherry, and it has undergone codon optimization. This composite part combines INP(30kda) and 8IIU(13.789kda), we succeeded in transferring this plasmid into Escherichia coli Nissle 1917 and let it express 8IIU. The plasmid uses lac promotor and has kanamycin resistence.
 
This work is derived from pUC19-8IIU and pBBR1MCS-INP-mCherry, and it has undergone codon optimization. This composite part combines INP(30kda) and 8IIU(13.789kda), we succeeded in transferring this plasmid into Escherichia coli Nissle 1917 and let it express 8IIU. The plasmid uses lac promotor and has kanamycin resistence.
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    <img src="https://static.igem.wiki/teams/5302/images/part-registry-p-inp-8iiu-1.png"
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        width="60%" style="display:block; margin:auto;" alt="Jamboree Program" >
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        <caption>
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            <b>Figure 1. </b> Colony PCR results of pBBR-INP-8IIU （1）
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    <img src="https://static.igem.wiki/teams/5302/images/part-registry-p-inp-8iiu-2.png"
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        width="60%" style="display:block; margin:auto;" alt="Jamboree Program" >
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    <div style="text-align:center;">
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        <caption>
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            <b>Figure 1. </b> Colony PCR results of pBBR-INP-8IIU （2）
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        </caption>
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    </div>
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    <img src="https://static.igem.wiki/teams/5302/images/part-registry-p-inp-8iiu-3.png"
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        width="60%" style="display:block; margin:auto;" alt="Jamboree Program" >
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    <div style="text-align:center;">
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        <caption>
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            <b>Figure 1. </b> Colony PCR results of pBBR-INP-8IIU （3）
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        </caption>
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 10:17, 1 October 2024


pBBR-INP-8IIU

This work is derived from pUC19-8IIU and pBBR1MCS-INP-mCherry, and it has undergone codon optimization. This composite part combines INP(30kda) and 8IIU(13.789kda), we succeeded in transferring this plasmid into Escherichia coli Nissle 1917 and let it express 8IIU. The plasmid uses lac promotor and has kanamycin resistence.

Jamboree Program
Figure 1. Colony PCR results of pBBR-INP-8IIU （1）

Jamboree Program
Figure 1. Colony PCR results of pBBR-INP-8IIU （2）

Jamboree Program
Figure 1. Colony PCR results of pBBR-INP-8IIU （3）

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 4325
    Illegal EcoRI site found at 4659
    Illegal XbaI site found at 3189
    Illegal PstI site found at 2016
    Illegal PstI site found at 3177
    Illegal PstI site found at 4427
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 4325
    Illegal EcoRI site found at 4659
    Illegal PstI site found at 2016
    Illegal PstI site found at 3177
    Illegal PstI site found at 4427
    Illegal NotI site found at 1057
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 4325
    Illegal EcoRI site found at 4659
    Illegal BglII site found at 1803
    Illegal BamHI site found at 3195
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 4325
    Illegal EcoRI site found at 4659
    Illegal XbaI site found at 3189
    Illegal PstI site found at 2016
    Illegal PstI site found at 3177
    Illegal PstI site found at 4427
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 4325
    Illegal EcoRI site found at 4659
    Illegal XbaI site found at 3189
    Illegal PstI site found at 2016
    Illegal PstI site found at 3177
    Illegal PstI site found at 4427
    Illegal NgoMIV site found at 2467
    Illegal NgoMIV site found at 2750
    Illegal NgoMIV site found at 3616
    Illegal NgoMIV site found at 5324
    Illegal AgeI site found at 5164
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1379
    Illegal SapI.rc site found at 2316
    Illegal SapI.rc site found at 2526