Difference between revisions of "Part:BBa K5302022"

 
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We used pBBR plasmid as a backbone and transfered V114 into Escherichia coli Nissle 1917, and finally succeeded in expressing V114.
 
We used pBBR plasmid as a backbone and transfered V114 into Escherichia coli Nissle 1917, and finally succeeded in expressing V114.
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    <img src="https://static.igem.wiki/teams/5302/images/part-registry-pov114-1.png"
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        width="60%" style="display:block; margin:auto;" alt="Jamboree Program" >
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            <b>Figure 1. </b> Colony PCR results of pBBR-OmpA-V114
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 10:10, 1 October 2024


pBBR-OmpA-V114

v114 has been identified as a small potent VEGF-binding peptide, which is composed of 8 amino acids--- that is VEPNc[CDIHVnLWEWEC]FERL, and there is a disulfide bond between N and F, making the stucture more stable. This peptide has a similiar structure and VEGF-binding site as V107, and it shows great affinity with VEGF(Kd=0.11 μM).

We used pBBR plasmid as a backbone and transfered V114 into Escherichia coli Nissle 1917, and finally succeeded in expressing V114.

Jamboree Program
Figure 1. Colony PCR results of pBBR-OmpA-V114

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 3979
    Illegal XbaI site found at 3140
    Illegal PstI site found at 1967
    Illegal PstI site found at 3128
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 3979
    Illegal PstI site found at 1967
    Illegal PstI site found at 3128
    Illegal NotI site found at 1008
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 3979
    Illegal BglII site found at 1754
    Illegal BamHI site found at 3146
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 3979
    Illegal XbaI site found at 3140
    Illegal PstI site found at 1967
    Illegal PstI site found at 3128
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 3979
    Illegal XbaI site found at 3140
    Illegal PstI site found at 1967
    Illegal PstI site found at 3128
    Illegal NgoMIV site found at 2418
    Illegal NgoMIV site found at 2701
    Illegal NgoMIV site found at 4637
    Illegal AgeI site found at 4477
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1330
    Illegal SapI.rc site found at 2267
    Illegal SapI.rc site found at 2477