Difference between revisions of "Part:BBa K5302019"

 
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We used pBBR plasmid as a backbone and transfered ZVEGF into Escherichia coli Nissle 1917, and finally succeeded in expressing ZVEGF.
 
We used pBBR plasmid as a backbone and transfered ZVEGF into Escherichia coli Nissle 1917, and finally succeeded in expressing ZVEGF.
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    <img src="https://static.igem.wiki/teams/5302/images/part-registry-pozvegf-1.png"
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            <b>Figure 1. </b> Colony PCR results of pBBR-OmpA-ZVEGF
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 10:01, 1 October 2024


pBBR-OmpA-ZVEGF

ZVEGF is a 59-residue three-helix peptide that was developed by Fedorova et al. by randomizing 9 residues on helices 1 and 2 of the Z-domain scaffold via phage display, followed by selection for binding to the VEGF8-109 dimer. A co-crystal structure of ZVEGF with VEGF8-109 shows that the engineered Z-domain adopts the expected three-helix bundle tertiary structure and engages the receptor-recognition sites on the VEGF dimer through a surface formed by helices 1 and 2 of ZVEGF. It shows great affinity with VEGF(Ki=0.41 μM).

We used pBBR plasmid as a backbone and transfered ZVEGF into Escherichia coli Nissle 1917, and finally succeeded in expressing ZVEGF.

Jamboree Program
Figure 1. Colony PCR results of pBBR-OmpA-ZVEGF

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 4100
    Illegal XbaI site found at 3140
    Illegal PstI site found at 1967
    Illegal PstI site found at 3128
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 4100
    Illegal PstI site found at 1967
    Illegal PstI site found at 3128
    Illegal NotI site found at 1008
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 4100
    Illegal BglII site found at 1754
    Illegal BamHI site found at 3146
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 4100
    Illegal XbaI site found at 3140
    Illegal PstI site found at 1967
    Illegal PstI site found at 3128
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 4100
    Illegal XbaI site found at 3140
    Illegal PstI site found at 1967
    Illegal PstI site found at 3128
    Illegal NgoMIV site found at 2418
    Illegal NgoMIV site found at 2701
    Illegal NgoMIV site found at 4758
    Illegal AgeI site found at 3789
    Illegal AgeI site found at 4598
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1330
    Illegal SapI.rc site found at 2267
    Illegal SapI.rc site found at 2477