Difference between revisions of "Part:BBa K5302015"

 
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This work is derived from pBBR1MCS-2 and mCherry-middle, and it has undergone codon optimization. This composite part combines AIDA(approximately 63.8kda) and mCherry(26.7kda), and we succeeded in transferring this plasmid into Escherichia coli Nissle 1917 and let it express mCherry as a fluorescence labeling.The plasmid uses lac promotor and has kanamycin resistence.
 
This work is derived from pBBR1MCS-2 and mCherry-middle, and it has undergone codon optimization. This composite part combines AIDA(approximately 63.8kda) and mCherry(26.7kda), and we succeeded in transferring this plasmid into Escherichia coli Nissle 1917 and let it express mCherry as a fluorescence labeling.The plasmid uses lac promotor and has kanamycin resistence.
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    <img src="https://static.igem.wiki/teams/5302/images/part-registry-pam-1.png"
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        width="60%" style="display:block; margin:auto;" alt="Jamboree Program" >
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    <div style="text-align:center;">
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        <caption>
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            <b>Figure 1. </b> structure of AIDA, from genebank
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        </caption>
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<div style="text-align:center;">
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    <img src="https://static.igem.wiki/teams/5302/images/part-registry-pam-2.png"
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        width="60%" style="display:block; margin:auto;" alt="Jamboree Program" >
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    <div style="text-align:center;">
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        <caption>
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            <b>Figure 2. </b> Colony PCR results of pBBR1MCS-AIDA-mCherry
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        </caption>
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 09:42, 1 October 2024


pBBR-AIDA-mCherry

This work is derived from pBBR1MCS-2 and mCherry-middle, and it has undergone codon optimization. This composite part combines AIDA(approximately 63.8kda) and mCherry(26.7kda), and we succeeded in transferring this plasmid into Escherichia coli Nissle 1917 and let it express mCherry as a fluorescence labeling.The plasmid uses lac promotor and has kanamycin resistence.

Jamboree Program
Figure 1. structure of AIDA, from genebank

Jamboree Program
Figure 2. Colony PCR results of pBBR1MCS-AIDA-mCherry

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 3610
    Illegal EcoRI site found at 3658
    Illegal EcoRI site found at 3931
    Illegal EcoRI site found at 4531
    Illegal EcoRI site found at 5227
    Illegal XbaI site found at 3421
    Illegal PstI site found at 2016
    Illegal PstI site found at 3919
    Illegal PstI site found at 4579
    Illegal PstI site found at 5165
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 3610
    Illegal EcoRI site found at 3658
    Illegal EcoRI site found at 3931
    Illegal EcoRI site found at 4531
    Illegal EcoRI site found at 5227
    Illegal PstI site found at 2016
    Illegal PstI site found at 3919
    Illegal PstI site found at 4579
    Illegal PstI site found at 5165
    Illegal NotI site found at 1057
    Illegal NotI site found at 4303
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 3610
    Illegal EcoRI site found at 3658
    Illegal EcoRI site found at 3931
    Illegal EcoRI site found at 4531
    Illegal EcoRI site found at 5227
    Illegal BglII site found at 1803
    Illegal BglII site found at 3414
    Illegal BamHI site found at 5429
    Illegal XhoI site found at 4276
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 3610
    Illegal EcoRI site found at 3658
    Illegal EcoRI site found at 3931
    Illegal EcoRI site found at 4531
    Illegal EcoRI site found at 5227
    Illegal XbaI site found at 3421
    Illegal PstI site found at 2016
    Illegal PstI site found at 3919
    Illegal PstI site found at 4579
    Illegal PstI site found at 5165
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 3610
    Illegal EcoRI site found at 3658
    Illegal EcoRI site found at 3931
    Illegal EcoRI site found at 4531
    Illegal EcoRI site found at 5227
    Illegal XbaI site found at 3421
    Illegal PstI site found at 2016
    Illegal PstI site found at 3919
    Illegal PstI site found at 4579
    Illegal PstI site found at 5165
    Illegal NgoMIV site found at 2467
    Illegal NgoMIV site found at 2750
    Illegal NgoMIV site found at 6052
    Illegal AgeI site found at 4137
    Illegal AgeI site found at 5892
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1379
    Illegal SapI.rc site found at 2316
    Illegal SapI.rc site found at 2526