Difference between revisions of "Part:BBa K5301021"

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We used this pathway to express SnTST- mCh[11] by using T7 expression system in <i>E.coli</i>, and the expression of SnTST- mCh[11] can be induced by IPTG. For more information on SnTST- mCh[11], go to <partinfo>BBa_K5301007</partinfo>.
 
We used this pathway to express SnTST- mCh[11] by using T7 expression system in <i>E.coli</i>, and the expression of SnTST- mCh[11] can be induced by IPTG. For more information on SnTST- mCh[11], go to <partinfo>BBa_K5301007</partinfo>.
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===Sequence and Features===
  
 
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K5301021 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K5301021 SequenceAndFeatures</partinfo>
  

Latest revision as of 09:38, 1 October 2024


SnTST-mCh[11] expression pathway (T7)

This pathway expresses SnTST- mCh[11] using T7 expression system.

Usage and Biology

We used this pathway to express SnTST- mCh[11] by using T7 expression system in E.coli, and the expression of SnTST- mCh[11] can be induced by IPTG. For more information on SnTST- mCh[11], go to BBa_K5301007.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1002
    Illegal NotI site found at 815
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 334
    Illegal BglII site found at 706
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]