Difference between revisions of "Part:BBa K5301027"
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We used this pathway to express sGFP11 tether(mSA - sGFP11) by using T7 expression system in <i>E.coli</i>, and the expression of sGFP11 tether can be induced by IPTG. For more information on sGFP11 tether[1-10], go to <partinfo>BBa_K5301018</partinfo>. | We used this pathway to express sGFP11 tether(mSA - sGFP11) by using T7 expression system in <i>E.coli</i>, and the expression of sGFP11 tether can be induced by IPTG. For more information on sGFP11 tether[1-10], go to <partinfo>BBa_K5301018</partinfo>. | ||
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Latest revision as of 09:30, 1 October 2024
sGFP11 tether expression pathway (T7)
This pathway expresses sGFP11 tether (BBa_K5301018) using T7 expression system,containing T7 promoter, mSA, sGFP11, and T7 terminator.
Usage and Biology
We used this pathway to express sGFP11 tether(mSA - sGFP11) by using T7 expression system in E.coli, and the expression of sGFP11 tether can be induced by IPTG. For more information on sGFP11 tether[1-10], go to BBa_K5301018.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 151
Illegal AgeI site found at 211 - 1000COMPATIBLE WITH RFC[1000]