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===Functional Parameters===
 
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Revision as of 09:12, 1 October 2024


spNW15 expression pathway (T7)

This pathway expresses the SpyCatcher-NW15-SpyTag fusion protein under IPTG induction, achieving self-cyclization of NW15 to construct nanodiscs.


Usage and Biology

We used this pathway to express spNW15 by using T7 expression system in E.coli, and the expression of spNW15 can be induced by IPTG. For more information on spNW15, go to BBa_K5301013.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 571
    Illegal SpeI site found at 523
    Illegal PstI site found at 739
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 571
    Illegal NheI site found at 139
    Illegal NheI site found at 1175
    Illegal SpeI site found at 523
    Illegal PstI site found at 739
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 571
    Illegal BglII site found at 627
    Illegal BglII site found at 999
    Illegal BamHI site found at 172
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 571
    Illegal SpeI site found at 523
    Illegal PstI site found at 739
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 571
    Illegal SpeI site found at 523
    Illegal PstI site found at 739
    Illegal AgeI site found at 1102
  • 1000
    COMPATIBLE WITH RFC[1000]