Difference between revisions of "Part:BBa K201004:Design"

(→‎Design Notes)
 
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This was done to allow an easier cloning of the operator sequence that is short in length (about 20 bp).
 
This was done to allow an easier cloning of the operator sequence that is short in length (about 20 bp).
  
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For details on the operator design go to [https://parts.igem.org/Part:BBa_K079019 BBa_K079019] page
 
===Source===
 
===Source===
  

Latest revision as of 02:22, 22 October 2009

Lac O2 natural operator with RFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 582
    Illegal AgeI site found at 694
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part was designed with the same structure of the Berkley constitutive promoters. Indeed, the operator sequence was digested with SpeI and PstI and the same was done for the RFP. The two parts were then ligated. This was done to allow an easier cloning of the operator sequence that is short in length (about 20 bp).

For details on the operator design go to BBa_K079019 page

Source

All subparts come from the Registry of Standard Parts

References