Difference between revisions of "Part:BBa K5499001"

Revision as of 08:35, 1 October 2024

CMV promotor

CMV promoters are often included in vectors used in genetic engineering work to enhance the expression level of the target gene.

Sequence and Features

Profile

Name: CMV Promoter
Origins: Derived from Human Cytomegalovirus (HCMV), a widely used promoter in mammalian cell research.
Properties:
The CMV promoter is a strong, constitutive promoter that drives high levels of gene expression in a variety of mammalian cells.
It is non-tissue-specific, meaning it functions efficiently in most mammalian cell lines, including human, rodent, and primate cells.
Often used in combination with the CMV enhancer to further boost transcription levels, it is particularly suited for experiments requiring high gene expression, such as recombinant protein production or reporter gene assays.
Due to its robust and stable expression characteristics, the CMV promoter is highly valued in iGEM projects.

Usage and Biology

The CMV promoter is one of the most commonly used promoters for driving high-level gene expression in mammalian cells. It is derived from the human cytomegalovirus (HCMV) immediate-early gene, which is highly active in a wide variety of cell types. The CMV promoter is constitutive, meaning it drives continuous and high transcriptional activity, making it ideal for applications such as gene therapy, recombinant protein production, and creating stable cell lines. Its robust performance in transient transfection experiments is also well-documented.
The CMV promoter is part of the human cytomegalovirus’s immediate-early regulatory region. It includes binding sites for various transcription factors, allowing for efficient transcription initiation in diverse cell types, particularly in mammalian systems. The promoter is often enhanced by elements such as the CMV enhancer(documented in BBa K5499001) to further increase its transcriptional strength.