Difference between revisions of "Part:BBa K187366"
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This is the reverse primer of a pair of universal primers for amplifying any gene out of pAB (BBa_187000). These primers contain uracil deoxyribonucleotides, allowing for digestion with the USER system which can be purchased from New England Biolabs (NEB). This produces A and B overhangs and finishes the production of the Bytes. With the gene contained within the pAB and pBA plasmids, Bytes can be easily stored and amplified using the universal primers at any time. | This is the reverse primer of a pair of universal primers for amplifying any gene out of pAB (BBa_187000). These primers contain uracil deoxyribonucleotides, allowing for digestion with the USER system which can be purchased from New England Biolabs (NEB). This produces A and B overhangs and finishes the production of the Bytes. With the gene contained within the pAB and pBA plasmids, Bytes can be easily stored and amplified using the universal primers at any time. | ||
| − | See the BioBytes RFC for details of the BioBytes assembly method. | + | See the BioBytes RFC for details of the BioBytes assembly method. Additional information in regards to characterization can be found at http://2009.igem.org/Team:Alberta/Optimization. |
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Latest revision as of 02:21, 22 October 2009
pAB_R (B end) USER primer for BioBytes plasmid pAB
This is the reverse primer of a pair of universal primers for amplifying any gene out of pAB (BBa_187000). These primers contain uracil deoxyribonucleotides, allowing for digestion with the USER system which can be purchased from New England Biolabs (NEB). This produces A and B overhangs and finishes the production of the Bytes. With the gene contained within the pAB and pBA plasmids, Bytes can be easily stored and amplified using the universal primers at any time.
See the BioBytes RFC for details of the BioBytes assembly method. Additional information in regards to characterization can be found at http://2009.igem.org/Team:Alberta/Optimization.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 16
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 16
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 16
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 16
- 1000COMPATIBLE WITH RFC[1000]