Difference between revisions of "Part:BBa K187365"

Latest revision as of 02:20, 22 October 2009

pAB_F (A end) USER primer for BioBytes plasmid pAB

This is the forward primer of a pair of universal primers for amplifying any gene out of pAB (BBa_K187000). These primers contain uracil deoxyribonucleotides, allowing for digestion with the USER system which can be purchased from New England Biolabs (NEB). This produces A and B overhangs and finishes the production of the Bytes. With the gene contained within the pAB and pBA plasmids, Bytes can be easily stored and amplified using the universal primers at any time.

See the BioBytes RFC for details of the BioBytes assembly method. Additional information in regards to characterization can be found at http://2009.igem.org/Team:Alberta/Optimization.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

Revision as of 18:31, 21 October 2009 (view source) Dridgway (Talk \| contribs) ← Older edit		Latest revision as of 02:20, 22 October 2009 (view source) Rpgguardian (Talk \| contribs)
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	This is the forward primer of a pair of universal primers for amplifying any gene out of pAB (<partinfo>BBa_K187000</partinfo>). These primers contain uracil deoxyribonucleotides, allowing for digestion with the USER system which can be purchased from New England Biolabs (NEB). This produces A and B overhangs and finishes the production of the Bytes. With the gene contained within the pAB and pBA plasmids, Bytes can be easily stored and amplified using the universal primers at any time.		This is the forward primer of a pair of universal primers for amplifying any gene out of pAB (<partinfo>BBa_K187000</partinfo>). These primers contain uracil deoxyribonucleotides, allowing for digestion with the USER system which can be purchased from New England Biolabs (NEB). This produces A and B overhangs and finishes the production of the Bytes. With the gene contained within the pAB and pBA plasmids, Bytes can be easily stored and amplified using the universal primers at any time.

−	See the BioBytes RFC for details of the BioBytes assembly method.	+	See the BioBytes RFC for details of the BioBytes assembly method. Additional information in regards to characterization can be found at http://2009.igem.org/Team:Alberta/Optimization.